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Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody [rBLCAM/6749]

In Stock
Catalog Number Formulation Size Price
933-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
933-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
933-MSM9-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody

IHC analysis of formalin-fixed, paraffin-embedded human tonsil using rBLCAM/6749 at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. Inset: PBS instead of primary antibody, secondary negative control.

Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/6749).

Recombinant CD22 / BL-CAM (B-Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified CD22 Recombinant Mouse Monoclonal Antibody (rBLCAM/6749). Confirmation of Purity and Integrity of Antibody.

CD22 Antibody in Western Blot (WB)

CD22 Antibody in Western Blot (WB)

Western Blot analysis of Human Spleen and Ramos lysates using CD22 Recombinant Mouse Monoclonal Antibody. (rBLCAM/6749).

CD22 Antibody in Western Blot (WB)

CD22 Antibody in Western Blot (WB)

Western Blot analysis of Spleen tissue lysates of different species using CD22 Recombinant Mouse Monoclonal Antibody. (rBLCAM/6749).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
CD22
Positive Control
lymph node or spleen., Raji cells or human peripheral blood lymphocytes (hPBL). Human tonsil
Immunogen
Recombinant fragment (around aa52-178) of the human CD22 protein (exact sequence is proprietary)
Alternate Names
B-cell receptor CD22, SIGLEC-2|SIGLEC2

Database Links

Entrez Gene ID
933
SwissProt
P20273

Additional Information

Clone
rBLCAM/6749
Chromosome Location
19q13.12
Mol. Weight of Antigen
95.35kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All CD22 Antibodies

Functions

  • Most highly expressed siglec (sialic acid-binding immunoglobulin-like lectin) on B-cells that plays a role in various aspects of B-cell biology including differentiation, antigen presentation, and trafficking to bone marrow (PubMed:34330755, PubMed:8627166). Binds to alpha 2,6-linked sialic acid residues of surface molecules such as CD22 itself, CD45 and IgM in a cis configuration. Can also bind to ligands on other cells as an adhesion molecule in a trans configuration (PubMed:20172905). Acts as an inhibitory coreceptor on the surface of B-cells and inhibits B-cell receptor induced signaling, characterized by inhibition of the calcium mobilization and cellular activation. Mechanistically, the immunoreceptor tyrosine-based inhibitory motif domain is phosphorylated by the Src kinase LYN, which in turn leads to the recruitment of the protein tyrosine phosphatase 1/PTPN6, leading to the negative regulation of BCR signaling (PubMed:8627166). If this negative signaling from is of sufficient strength, apoptosis of the B-cell can be induced (PubMed:20516366).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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