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Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody [CR2/7185R]

In Stock
Catalog Number Formulation Size Price
1380-RBM15-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1380-RBM15-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1380-RBM15-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/7185R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human tonsil stained with CD21 Recombinant Rabbit Monoclonal Antibody (CR2/7185R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

CR2 Antibody in SDS-PAGE

CR2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Complement receptor type 2 Recombinant Rabbit Monoclonal Antibody (CR2/7185R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD21 also known as complement C3d receptor, Epstein-Barr virus receptor, and complement receptor type 2 (CR2), is a protein that in humans is encoded by the CR2 gene. This protein is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. B cells have CR2 receptors on their surfaces, allowing the complement system to play a role in B-cell activation and maturation.It is expressed by follicular dendritic cells (FDC) and mature B cells, as well as by several types of epithelial cells. CD21 is useful in the identification of follicular dendritic cell matrix found in normal lymph node and tonsillar tissue, and also useful in identifying abnormal.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CR2
Positive Control
Human tonsil tissue.
Immunogen
Recombinant fragment (around aa1020-1033) of the human CD21 protein (exact sequence is proprietary)
Alternate Names
Complement receptor type 2, C3DR|CD21|CR|CVID7|SLEB9

Database Links

Entrez Gene ID
1380
SwissProt
P20023

Additional Information

Clone
CR2/7185R
Chromosome Location
1q32.2
Mol. Weight of Antigen
112.91kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All CR2 Antibodies

Functions

  • Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1 (PubMed:1849076, PubMed:21527715, PubMed:7753047). When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells (PubMed:21527715). This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling (PubMed:1383329, PubMed:30107486). Also induces specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner (PubMed:12938232). Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production (PubMed:1386409)., (Microbial infection) Acts as a receptor for Epstein-Barr virus.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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