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Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody [LFA2/8845R]

Additional information

Catalog Number: 914-RBM23
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
914-RBM23-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
914-RBM23-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
914-RBM23-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody

SDS-PAGE Analysis of Purified CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/8845R). Confirmation of Purity and Integrity of Antibody.

Recombinant CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 2

Formalin-fixed, paraffin-embedded human lymph node stained with CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/8845R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

CD2 Antibody in Western Blot (WB)

CD2 Antibody in Western Blot (WB)

Western Blot Analysis of MOLT-4 cell lysate using CD2 Recombinant Rabbit Monoclonal Antibody (LFA2/8845R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
LFA2/8845R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD2
Positive Control
Human tonsil|spleen, lymph node, or MOLT-4
Immunogen
Recombinant fragment (around aa272-374) of the human CD2 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
914
SwissProt
P06729
Chromosome Location
1p13.1
Mol. Weight of Antigen
39.45kDa
Alternate Names
T-cell surface antigen CD2, LFA-2|SRBC|T11

Summary

CD2 is mainly present on the surface of mature T cells and NK cell membranes, while B cells are usually not expressed. It can be used to label the diagnosis and study of normal T cells and their associated tumors, such as pre-t cell lymphoma, marginal T cell lymphoma and anaplastic cell lymphoma. CD2 antibody is useful for identification of precursor and mature T-cell lymphomas. Aberrant loss of CD2 in T-cell lymphomas may help to distinguish them from reactive T-cell proliferations.

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