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Recombinant CD1a / HTA1 (Mature Langerhans Cells Marker) Antibody [MSVA-001M]

Additional information

Catalog Number: 909-MSM21
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
909-MSM21-P0
Purified Ab with BSA and Azide
20ug
$324.50
909-MSM21-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD1A Antibody in Numerous Tissues (IHC)

CD1A Antibody in Numerous Tissues (IHC)

T-cell surface glycoprotein CD1a Mouse Recombinant Monoclonal Antibody (MSVA-001M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-001M
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Endosome membrane, Membrane raft
Gene Name
CD1A
Positive Control
Skin: A strong CD1a staining should be seen in Langerhans cells.
Immunogen
Recombinant full-length human CD1a protein
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
Key References
  • McNally, AK.et al.2011. Exp. Mol. Pathol. 91: 673-681.
  • Matsuda, A.et al.2009. Invest. Ophthalmol. Vis. Sci. 50: 2871-2877.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
909
SwissProt
P06126
Chromosome Location
1q23.1
Mol. Weight of Antigen
49kDa
Alternate Names
T-cell surface glycoprotein CD1a, T-cell surface antigen T6/Leu-6, Cortical thymocyte antigen CD1A, Epidermal dendritic cell marker CD1a antibody, FCB6, HTA1, T cell surface antigen T6 / Leu 6, T-Cell Surface Glycoprotein CD1A

Summary

At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T cell response to non-peptide lipid and glycolipid antigens and play a role in non-classical antigen presentation. CD1a is a non-polymorphic MHC Class 1 related cell surface glycoprotein, expressed in association with Beta-2 microglobulin. Anti-CD1a labels Langerhans cell histiocytosis (Histiocytosis X), extranodal histiocytic sarcoma, a subset of T-lymphoblastic lymphoma/leukemia, and interdigitating dendritic cell sarcoma of the lymph node. When combined with antibodies against TTF-1 and CD5, anti-CD1a is useful in distinguishing between pulmonary and thymic neoplasms since CD1a is consistently expressed in thymic lymphocytes in both typical and atypical thymomas, but only focally in 1/6 of thymic carcinomas and not in lymphocytes in pulmonary neoplasms. Anti-CD1a is reported to be a new marker for perivascular epithelial cell tumor (PEComa).

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