Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody [rCALD1/7266]

Additional information

Catalog Number: 800-MSM16
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
800-MSM16-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
800-MSM16-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
800-MSM16-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC
  • WB

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Host
Mouse
Species Reactivity
Clone
rCALD1/7266
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytoskeleton, Myofibril, Stress fiber
Gene Name
Positive Control
Blood vessels in all tissues, smooth muscle or leiomyosarcoma. Skeletal muscle.
Immunogen
Recombinant human full-length CALD1 protein
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
800
SwissProt
Chromosome Location
7q33
Mol. Weight of Antigen
93.23kDa
Alternate Names
Caldesmon, CDM|H-CAD|HCAD|L-CAD|LCAD|NAG22|h-CD

Summary

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

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