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Formalin-fixed, paraffin-embedded human Spleen stained with Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/796).
Western Blot Analysis of MCF-7 cell lysate using Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/796).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Bcl-2 Mouse Recombinant Monoclonal Antibody (rBCL2/796). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE Analysis of Purified Apoptosis regulator Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/796). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Heart, Human Skeletal muscle, Human Spleen, Jurkat, MCF7, Raji and THP1 lysates using Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/796).
Western Blot Analysis of Heart, Skeletal muscle and Spleen tissue lysates of different species using Bcl-2 Recombinant Mouse Monoclonal Antibody (rBCL2/796).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This antibody recognizes a protein of 25-26kDa, identified as the bcl-2lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
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