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Recombinant PHOX2B Antibody [PHOX2B/7161R]

Additional information

Catalog Number: 8929-RBM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
8929-RBM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
8929-RBM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
8929-RBM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant PHOX2B Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with PHOX2B Recombinant Rabbit Monoclonal Antibody (PHOX2B/7161R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant PHOX2B Antibody - Image 2

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with PHOX2B Recombinant Rabbit Monoclonal Antibody (PHOX2B/7161R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
PHOX2B/7161R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PHOX2B
Positive Control
brain and adrenal gland., Expressed in neuroblastoma
Immunogen
Recombinant fragment of human PHOX2B protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in the development of several major noradrenergic neuron populations, including the locus coeruleus. Transcription factor which could determine a neurotransmitter phenotype in vertebrates. Enhances second-messenger-mediated activation of the dopamine beta-hydrolase and c-fos promoters, and of several enhancers including cAMP-response element and serum-response element.
Key References
  • Johnson, K.R., et al. 1996. Mapping of the ARIX homeodomain gene to mouse chromosome 7 and human chromosome 11q13. Genomics 33:527-531.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
8929
SwissProt
Q99453
Chromosome Location
4p13
Mol. Weight of Antigen
32kDa
Alternate Names
Paired mesoderm homeobox protein 2B, Neuroblastoma Phox, PHOX2B homeodomain protein, Paired-like homeobox 2B, NBLST2; NBPhox; Neuroblastoma paired type homeobox protein; Neuroblastoma Phox; Paired like homeobox 2b; Paired mesoderm homeobox protein 2B; Paired-like homeobox 2B; PHOX2B homeodomain protein; PMX2B

Summary

Paired homeobox protein 2B (PHOX2B) is a transcription factor located on chromosome 4p13, which is essential for the formation of autonomic ganglia in the autonomic nervous system.PHOX2B is expressed in neuroblastoma, brain and adrenal glands. PHOX2B deficiency can lead to susceptibility to congenital central hypoventilation syndrome (CCHS) and neuroblastoma type 2 (NBLST2).

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