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Periostin (POSTN) Antibody [MSVA-649M]

In Stock
Catalog Number Formulation Size Price
10631-MSM16-P0
Purified Ab with BSA and Azide
20ug
$324.50
10631-MSM16-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
POSTN Antibody in Numerous Tissues (IHC)

POSTN Antibody in Numerous Tissues (IHC)

Periostin Mouse Monoclonal Antibody (MSVA-649M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for aVb3 and aVb5 Integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor b1 (TGFb1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Endothelial Cell Marker
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Golgi apparatus, Secreted
Gene Name
POSTN
Positive Control
Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness.
Immunogen
Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)
Alternate Names
Periostin, Osteoblast-specific factor 2, Fasciclin-I like; Osteoblast specific factor 2 (OSF2); PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2 / th4 / thy6 / thy8; Periostin osteoblast specific factor; PN; POSTN

Database Links

Entrez Gene ID
10631
SwissProt
Q15063

Additional Information

Clone
MSVA-649M
Chromosome Location
13q13.3
Mol. Weight of Antigen
84/74kDa (Periostin); 90kDa (secreted glycoprotein)

Related Antibodies

View All POSTN Antibodies

Functions

  • Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

Key References

  • Norris, R.A., et al. 2004. Identification and detection of the Periostin gene in cardiac development. Anat. Rec. A Discov. Mol. Cell. Evol. Biol.281: 1227-1233.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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