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Formalin-fixed, paraffin-embedded human tonsil stained with PAX3 Mouse Monoclonal Antibody (PAX3/8424). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human tonsil stained with PAX3 Mouse Monoclonal Antibody (PAX3/8424). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified Paired box protein Pax-3 Mouse Monoclonal Antibody (PAX3/8424). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Brain and Human Skeletal muscle lysates using PAX3 Mouse Monoclonal Antibody (PAX3/8424).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development. The product of the PAX3 gene is a DNA-binding protein expressed during early neurogenesis. Pax-3 is a protein containing both a paired domain and a paired-type homeodomain. During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon. In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme. Mutations in the MITF and Pax-3 genes, encoding transcription factors, are responsible for Waardenburg syndrome II (WSII) and WSI/WSIII, respectively.
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