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CDKN2A / p16INK4a / p14ARF Antibody [4C6/4]

Additional information

Catalog Number: 1029-MSM1
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1029-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1029-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1029-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CDKN2A / p16INK4a / p14ARF Antibody

Formalin-fixed, paraffin-embedded human Brain stained with p14ARF Mouse Monoclonal Antibody (4C6/4).

CDKN2A / p16INK4a / p14ARF Antibody - Image 2

Formalin-fixed, paraffin-embedded rat Brain stained with p14ARF Mouse Monoclonal Antibody (4C6/4).

CDKN2A / p16INK4a / p14ARF Antibody - Image 3

SDS-PAGE Analysis of Purified p14ARF Mouse Monoclonal Antibody (4C6/4). Confirmation of Integrity and Purity of Antibody.

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human, Rat
Clone
4C6/4
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
CDKN2A
Positive Control
H1299 and HeLa cells; human prostate carcinoma., MCF7
Immunogen
Recombinant full-length human p14 ARF protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Key References
  • Sherr, C.J. 1993. Mammalian G1 cyclins. Cell 73: 1059-1065.
  • Hunter, T. 1993. Braking the cycle. Cell 75: 839-841.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1029
SwissProt
P42771
Chromosome Location
9p21.3.
Mol. Weight of Antigen
14kDa
Alternate Names
Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, ARF; CDK4 inhibitor p16 INK4; CDK4I; CDKN2; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Cyclin dependent kinase inhibitor 2A; INK4; INK4a; Melanoma p16 inhibits CDK4; MLM; MTS 1; MTS1; Multiple tumor suppressor 1; p14; p16; p16 INK4a; p16INK4a; p19; P19ARF; TP16

Summary

The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the pro- duction of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.

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