Formalin-fixed, paraffin-embedded human Brain stained with p14ARF Mouse Monoclonal Antibody (4C6/4).

Formalin-fixed, paraffin-embedded rat Brain stained with p14ARF Mouse Monoclonal Antibody (4C6/4).

SDS-PAGE Analysis Purified p14ARF Mouse Monoclonal Antibody (4C6/4). Confirmation of Integrity and Purity of Antibody.

Printable Datasheet

p14ARF [4C6/4]

Name: p14ARF
SKU: 1029-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the pro- duction of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.

Product Properties & Targets

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Species Reactivity

Human, Rat

Research Areas

Cancer, Cardiovascular

Isotype

IgG2a

Cellular Localization

Cytoplasm, Nucleus

Light Chain

Kappa

Gene Name

CDKN2A

Positive Control

H1299 and HeLa cells; human prostate carcinoma., MCF7

Immunogen

Recombinant full-length human p14 ARF protein

Alternate Names

Cyclin-dependent kinase inhibitor 2A, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1, p16-INK4a, ARF; CDK4 inhibitor p16 INK4; CDK4I; CDKN2; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent kinase 4 inhibitor A; Cyclin dependent kinase inhibitor 2A; INK4; INK4a; Melanoma p16 inhibits CDK4; MLM; MTS 1; MTS1; Multiple tumor suppressor 1; p14; p16; p16 INK4a; p16INK4a; p19; P19ARF; TP16

Database Links

Entrez Gene ID

1029

Unigene

512599

SwissProt

P42771

Additional Information

Clone

4C6/4

Human Gene Symbol

ARF

Chromosome Location

9p21.3.

Mol. Weight of Antigen

14kDa

Functions

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Sherr, C.J. 1993. Mammalian G1 cyclins. Cell 73: 1059-1065.
Hunter, T. 1993. Braking the cycle. Cell 75: 839-841.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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