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OVOL2 / CRE-Bpa Antibody [OVOL2/6776]

Additional information

Catalog Number: 58495-MSM6
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
58495-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
58495-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
58495-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
OVOL2 / CRE-Bpa Antibody

Formalin-fixed, paraffin-embedded human colon carcinoma stained with OVOL2 Mouse Monoclonal Antibody (OVOL2/6776). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
OVOL2/6776
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Nucleus.
Gene Name
OVOL2
Positive Control
HeLa|U87 or K562 cells.
Immunogen
Recombinant fragment (around aa175-275) of human OVOL2 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Zinc-finger transcription repressor factor (PubMed:19700410). Plays a critical role in maintaining the identity of epithelial lineages by suppressing epithelial-to mesenchymal transition (EMT) mainly through the repression of ZEB1, an EMT inducer (By similarity). Positively regulates neuronal differentiation (By similarity). Suppresses cell cycling and terminal differentiation of keratinocytes by directly repressing MYC and NOTCH1 (PubMed:19700410). Important for the correct development of primordial germ cells in embryos (By similarity). Plays dual functions in thermogenesis and adipogenesis to maintain energy balance. Essential for brown/beige adipose tissue-mediated thermogenesis, is necessary for the development of brown adipocytes. In white adipose tissues, limits adipogenesis by blocking CEBPA binding to its transcriptional targets and inhibiting its transcription factor activity (By similarity).
Key References
  • Zhang, T., et al. The Journal of Biological Chemistry 288:6166-6177 (2013).
  • Nucleic Acids Res. 38:D142-D148 (2010).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
58495
SwissProt
Q9BRP0
Chromosome Location
20p11.23
Mol. Weight of Antigen
69.1kDa
Alternate Names
Transcription factor Ovo-like 2, Zinc finger protein 339, bA504H3.3; hOvo2; Ovo like 2; ovo-like zinc finger 2 (OVOL2); Transcription factor Ovo-like 2; Zinc finger protein 339 (ZNF339)

Summary

The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.

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