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Occludin (Marker of Early Blood Brain Barrier Damage) Antibody [MSVA-415M]

In Stock
Catalog Number Formulation Size Price
100506658-MSM29-P0
Purified Ab with BSA and Azide
20ug
$324.50
100506658-MSM29-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
OCLN Antibody in Numerous Tissues (IHC)

OCLN Antibody in Numerous Tissues (IHC)

Occludin Mouse Monoclonal Antibody (MSVA-415M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
BBB VCAM-1 Signaling, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Tight junction
Gene Name
OCLN
Positive Control
Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Immunogen
Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
Alternate Names
Occludin, BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin

Database Links

Entrez Gene ID
100506658
SwissProt
Q16625

Additional Information

Clone
MSVA-415M
Chromosome Location
5q13.2
Mol. Weight of Antigen
60-82kDa

Related Antibodies

View All OCLN Antibodies

Functions

  • May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

Key References

  • Pan, R. et al. Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke. Sci. Rep. 7, 40331; (2017)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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