Formalin-fixed, paraffin-embedded human neuroendocrine tumor stained with NSE gamma Mouse Monoclonal Antibody (ENO2/2706).

Formalin-fixed, paraffin-embedded human neuroendocrine tumor stained with NSE gamma Mouse Monoclonal Antibody (ENO2/2706).

Printable Datasheet

NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) [ENO2/2706]

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Summary

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.

Product Properties & Targets

Host
Mouse
Species Reactivity
Human
Isotype
IgG1
Cellular Localization
Cell membrane, Cytoplasm
Light Chain
kappa
Gene Name
ENO2
Positive Control
Cerebellum or Pheochromocytoma., HeLa or Y79 cells. Pancreas, HePG2, SH-SY-5Y
Immunogen
A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
Alternate Names
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE

Database Links

Entrez Gene ID
2026
Unigene
511915
SwissProt
P09104

Additional Information

Clone
ENO2/2706
Human Gene Symbol
ENO2
Chromosome Location
12p13
Mol. Weight of Antigen
~50kDa

Functions

  • Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Known Applications & Suggested Dilutions

  • ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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