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NOX4 / NADPH Oxidase 4 Antibody [NOX4/1245]

In Stock
Catalog Number Formulation Size Price
50507-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
50507-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
50507-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
NOX4 / NADPH Oxidase 4 Antibody

SDS-PAGE Analysis of Purified NOX4 Mouse Monoclonal Antibody (NOX4/1245). Confirmation of Integrity and Purity of Antibody

Applications & Dilutions

Applications Tested Dillution Protocol Note
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Hypoxia, BBB VCAM-1 Signaling, Infectious Disease
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell junction, Cell membrane, Endoplasmic reticulum membrane, Focal adhesion, Nucleolus, Nucleus
Gene Name
NOX4
Positive Control
JAR cells. Kidney., PC-12
Immunogen
Recombinant human NOX4 protein
Alternate Names
NADPH oxidase 4, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, Renal NAD(P)H-oxidase, Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX-1; NADPH oxidase 4; Nox4; Renal NAD(P)H-oxidase; RENOX

Database Links

Entrez Gene ID
50507
SwissProt
Q9NPH5

Additional Information

Clone
NOX4/1245
Chromosome Location
11q14.2-q21
Mol. Weight of Antigen
70kDa

Related Antibodies

View All NOX4 Antibodies

Functions

  • Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
  • Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.

Key References

  • Geiszt, M., et al. 2000. Proc. Natl. Acad. Sci. USA 97: 8010-8014.
  • Shiose, A., et al. 2001. J. Biol. Chem. 276: 1417-1423.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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