Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

Formalin-fixed, paraffin-embedded human Pancreas stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Milk Fat Globule Monoclonal Antibody (MFG-06)

SDS-PAGE Analysis Purified Milk Fat Globule Monoclonal Antibody (MFG-06). Confirmation of Integrity and Purity of Antibody

Milk Fat Globule (Breast Epithelial Marker) [MFG-06]

Name: Milk Fat Globule (Breast Epithelial Marker)
SKU: 4240-MSM1
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Printable

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

Acrosome membrane, Cytoplasmic vesicle, Membrane, Secreted, Secretory vesicle

Gene Name

MFGE8

Mol. Weight of Antigen

45kDa

Immunogen

Human milk fat globule membrane preparation

Database Links

Entrez Gene ID

4240

Unigene

3745

SwissProt

Q08431

Additional Information

Clone

MFG-06

Human Gene Symbol

MFGE8

Chromosome Location

15q25

Known Applications & Suggested Dilutions

Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Key References

Larocca D et al. A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains. Cancer Res 1991, 51: 4994-4998
Ceriani RL et al. Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci USA 1977, 74(2):582-6
Corcoran D and Walker RA. Ultrastructural localization of milk fat globule membrane antigens in human breast carcinomas. J Pathol 1990,161(2):161-6

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.