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Migfilin-1 / FBLIM1 Antibody [FBLIM1/4600]

In Stock
Catalog Number Formulation Size Price
54751-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
54751-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
54751-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cytoplasm, Cytoskeleton, Focal adhesion, Stress fiber
Gene Name
Positive Control
A431 or MCF7 cells. Human heart or kidney tissue.
Immunogen
Recombinant full-length human FBLIM1 protein
Alternate Names
Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein, CSX associated LIM (Cal); Filamin binding LIM protein 1 (FBLIM1); Filamin-binding LIM protein 1 (FBLP1); Gt10; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
FBLIM1/4600
Chromosome Location
14q22.1
Mol. Weight of Antigen
~41kDa

Functions

  • Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion.

Key References

  • Yizeng et al. Cell (2003) 113(1): 37-47.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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