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Mesothelin (Mesothelial Marker) Antibody [MSVA-235M]

In Stock
Catalog Number Formulation Size Price
10232-MSM22-P0
Purified Ab with BSA and Azide
20ug
$269.50
10232-MSM22-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
MSLN Antibody in Numerous Tissues (IHC)

MSLN Antibody in Numerous Tissues (IHC)

Mesothelin Mouse Monoclonal Antibody (MSVA-235M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cell membrane, Golgi apparatus, Secreted
Gene Name
MSLN
Positive Control
Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.
Immunogen
Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)
Alternate Names
Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, CAK1; Megakaryocyte potentiating factor; Mesothelin; MSLN; SMR; SMRP

Database Links

Entrez Gene ID
10232
SwissProt
Q13421

Additional Information

Clone
MSVA-235M
Chromosome Location
16p13.3
Mol. Weight of Antigen
40kDa (Mature form); 69kDa (Precursor)

Related Antibodies

View All MSLN Antibodies

Functions

  • Membrane-anchored forms may play a role in cellular adhesion.
  • Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Key References

  • Chang, K. and Pastan, I. 1996. Molecular cloning of Mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab purified from Bioreactor Concentrate by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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