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MCM7 (Proliferation Marker) Antibody [MCM7/1466]

In Stock
Catalog Number Formulation Size Price
4176-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4176-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4176-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Western Blot Analysis A) HeLa (B) Raji and C) HepG2 cell lysate Using MCM7 Mouse Monoclonal Antibody (MCM7/1466).

Western Blot Analysis A) HeLa (B) Raji and C) HepG2 cell lysate Using MCM7 Mouse Monoclonal Antibody (MCM7/1466).

Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Mouse Monoclonal Antibody (MCM7/1466).

Formalin-fixed, paraffin-embedded human Tonsil stained with MCM7 Mouse Monoclonal Antibody (MCM7/1466).

SDS-PAGE Analysis of Purified MCM7 Mouse Monoclonal Antibody (MCM7/1466). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified MCM7 Mouse Monoclonal Antibody (MCM7/1466). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of MCF-7, HEK-293, A549 lysate using MCM7 Mouse Monoclonal Antibody (MCM7/1466).

Western Blot Analysis of MCF-7, HEK-293, A549 lysate using MCM7 Mouse Monoclonal Antibody (MCM7/1466).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Dog, Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MCM7
Positive Control
A549, HEK-293, MCF-7, Raji or Ramos cells. Tonsil.
Immunogen
Recombinant human MCM7 protein fragment (aa195-319)
Alternate Names
DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3, CDC47; DNA replication licensing factor MCM7; MCM7 mini chromosome maintenance deficient 7; Mini chromosome Maintenance 7; Mini chromosome maintenance protein 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146

Database Links

Entrez Gene ID
4176
SwissProt
P33993

Additional Information

Clone
MCM7/1466
Chromosome Location
7q21.3-q22.1
Mol. Weight of Antigen
88kDa

Related Antibodies

View All MCM7 Antibodies

Functions

  • Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.

Key References

  • Koonin, E.V. 1993. Nucleic Acids Res. 21: 2541-2547.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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