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Kindlin-1 / KIND1 Antibody [4A5.14]

Additional information

Catalog Number: 55612-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
55612-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
55612-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
55612-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Kindlin-1 / KIND1 Antibody

Formalin-fixed, paraffin-embedded human liver stained with KIND1 Mouse Monoclonal Antibody (4A5.14).

Kindlin-1 / KIND1 Antibody - Image 2

SDS-PAGE Analysis of Purified KIND1 Mouse Monoclonal Antibody (4A5.14). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
4A5.14
Isotype
IgG2a
Light Chain
Lambda
Cellular Localization
Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Focal adhesion, Ruffle membrane
Gene Name
FERMT1
Positive Control
293T whole cell lysate. Human tonsil or liver.
Immunogen
Recombinant protein containing Kindlin-1 (residues 216-677)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in cell adhesion. Contributes to integrin activation. When coexpressed with talin, potentiates activation of ITGA2B. Required for normal keratinocyte proliferation. Required for normal polarization of basal keratinocytes in skin, and for normal cell shape. Required for normal adhesion of keratinocytes to fibronectin and laminin, and for normal keratinocyte migration to wound sites. May mediate TGF-beta 1 signaling in tumor progression.
Key References
  • Histopathology (2007) 51(4):499-508.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
55612
SwissProt
Q9BQL6
Chromosome Location
20p12.3
Mol. Weight of Antigen
77kDa
Alternate Names
Fermitin family homolog 1, Kindlerin, Kindlin syndrome protein, Kindlin-1, Unc-112-related protein 1, Fermitin family homolog 1 (Fermt1); Kindlin-1 (KIND1); Kindlerin; Unc-112; UNC112A; URP1; DTGCU2

Summary

Kindlin-1 (also known as KIND1, KINDLERIN, UNC112-RELATED PROTEIN 1 (URP1) is an important component of cell-extracellular matrix adhesion. Kindlin-1 is involved in cell adhesion, possibly via its interaction with integrins and may mediate TGF-beta 1 signaling during tumor progression. Kindlin-1 has been shown to be significantly upregulated in lung and colon carcinomas. Mutations in the kindlin-1 gene, FERMT1, causes Kindler syndrome. Kindler syndrome is an autosomal recessive disorder characterized by neonatal blistering, sun sensitivity, atrophy, abnormal pigmentation and fragility of the skin. We have developed monoclonal anti-kidlin-1 antibodies. This monoclonal antibody recognizes kindlin-1 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

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