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Kappa Light Chain (B-Cell Marker) Antibody [HP6053 + L1C1]

Additional information

Catalog Number: 3514-MSM5
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3514-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3514-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3514-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Kappa Light Chain (B-Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Kappa Light Chain Mouse Monoclonal Antibody (HP6053 + L1C1).

Kappa Light Chain (B-Cell Marker) Antibody - Image 2

Western Blot Analysis of Raji cell lysate using Kappa Light Chain Mouse Monoclonal Antibody (HP6053 + L1C1).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
HP6053 + L1C1
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
IGKV1D-16
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Immunogen
Purified human Ig kappa chain (HP6053) & Human B-Lymphoma Cells (L1C1)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).
Key References
  • Takahashi H et. al. Pathol Res Prac 189:300-311 (1993).2. Momose H et. al. Hum Pathol. 23:1115-1119 (1992).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3514
SwissProt
P01601
Chromosome Location
2p11.2
Mol. Weight of Antigen
~22.5kDa
Alternate Names
Immunoglobulin kappa variable 1D-16, Ig kappa chain V-I region HK146, Ig kappa chain V-I region HK189, HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM

Summary

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

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