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Interleukin-15 (IL-15) Antibody [IL15/4355]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
3600-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3600-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3600-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Interleukin-15 (IL-15), also designated IL-T, is a cloned cytokine which shares several biological activities but no sequence homology with IL-2. Human, mouse and simian IL-15 cDNA clones have been isolated and characterized. All 3 species encode a 162 amino acid residue precursor protein containing a 48 amino acid leader that is cleaved to generate the mature form of IL-15. IL-15 stimulates the proliferation of T cells and NK cells, while enhancing B cell expansion and antibody production. Unlike IL-2, IL-15 is not produced by lymphocytes, but appears to be produced by macrophages, epithelial lines, muscle and placenta. IL-15 has also been shown to be a chemoattractant for human blood T lymphocytes and to be able to induce lymphokine-activated killer (LAK) activity in NK cells as well as to be able to induce the generation of cytolytic effector cells. Studies have shown that IL-15 is the only other cytokine that shares the 尾 signaling subunit of the IL-2R. Evidence also suggests that like IL-2, IL-4 and IL-7, IL-15 utilizes the common IL-2R纬 subunit.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
Positive Control
Human placenta, skeletal muscle or adrenal gland.
Immunogen
Recombinant fragment of human IL15 (exact sequence is proprietary)
Alternate Names
Interleukin-15, Interleukin-15 (IL-15); MGC9721

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
IL15/4355
Chromosome Location
4q31.21
Mol. Weight of Antigen
14-15kDa

Functions

  • Cytokine that stimulates the proliferation of T-lymphocytes (PubMed:8178155). Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA (PubMed:8178155). In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation (PubMed:15123770).

Key References

  • Wang, Hongsheng et al. The Journal of investigative dermatology vol. 140,9 (2020): 1824-1836.e7. doi:10.1016/j.jid.2020.01.026
  • Reche PA, et al. Journal of Immunology. 2001;167(1):336 343. doi:10.4049/jimmunol.167.1.336

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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