| Catalog Number: | MSM1-1331 |
|---|---|
| Reactivity: | |
| Gene: | |
| Host: |
Trial Size Available
Carrier-Free Available
SDS-PAGE Analysis Purified IgG1 Isotype Control for Mouse Monoclonal Abs (ICG1/ 1331). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human heart stained with IgG1 Isotype Control for Mouse Monoclonal Abs (ICG1/ 1331). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.
Confocal immunofluorescence image of PFA-fixed Raji cells stained with IgG1 Negative Control Mouse Monoclonal Antibody (ICG1/ 1331) followed by goat anti-mouse CF488 (no staining). RedDot is used to label the nuclei red.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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To serve as a true negative control for various procedures, this mouse monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
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