Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).

SDS-PAGE Analysis Purified Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4). Confirmation of Integrity and Purity of Antibody.

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunofluorescent staining of HeLa cells using Histone H1 Mouse Monoclonal Antibody (rAE-4) followed by goat anti-Mouse IgG conjugated to CF488 (green). Phalloidin is used to label cellmembrane (red).

Western Blot Analysis of human heart tissue lysate using Histone H1 Mouse Recombinant Monoclonal Antibody (rAE-4).

Printable Datasheet

Histone H1 (Pan Nuclear Marker) [rAE-4]

Name: Histone H1 (Pan Nuclear Marker)
SKU: 3005-MSM8
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies Recombinant Mouse Monoclonals Recombinants

Summary

Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a purer preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format.Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Isotype

IgG2a

Cellular Localization

N/A

Gene Name

H1-0, N/A

Mol. Weight of Antigen

30kDa

Immunogen

Recombinant full-length human Histone H1 protein

Database Links

Entrez Gene ID

3005

Unigene

226117; 97358

SwissProt

N/A

Additional Information

Clone

rAE-4

Human Gene Symbol

Chromosome Location

3q21-q22; 6q21-q22; 22q13.1

Known Applications & Suggested Dilutions

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.