Recombinant HBsAg (Hepatitis B surface Ag a determinant) [HBsAg/7555R]

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Summary

Hepatitis B surface antigen (HBsAg) is a glycoprotein on the surface of hepatitis B virus. �After hepatitis B infection, HBsAg appears as the first viral marker. �HBsAg can be detected in blood, saliva, breast milk, sweat, tears, nasopharyngeal secretions, semen and vaginal secretions of patients 2 to 6 months after infection with HBV. �HBsAg antibodies are mainly used for the diagnosis of hepatitis B virus.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
Cytoplasm.
Positive Control
HBV infected liver.
Immunogen
Recombinant full-length human HBsAg protein
Alternate Names
, HBsAg; HBV major surface antigen; HBV surface antigen; Hepatitis B Virus major surface antigen; Major surface antigen; S antibody

Database Links

Entrez Gene ID
Unigene
N/A
SwissProt

Additional Information

Clone
HBsAg/7555R
Human Gene Symbol
HBsAg
Chromosome Location
N/A
Mol. Weight of Antigen
42kDa

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&deg
  • C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Aden, D.P., et al. 1979. Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282: 615-616.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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