HA Tag [HA/279]

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Summary

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
60kDa
Immunogen
HA-molecule corresponding to amino acids 98-106 (YPYDVPDYA).

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
HA/279
Human Gene Symbol
Not Known
Chromosome Location
Not Known

Known Applications & Suggested Dilutions

  • ELISA (For coating, order antibody without BSA)
  • Immunofluorescence (1-2ug/ml)
  • Western Blot (1-2ug/ml)
  • ,Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate))
  • Flow Cytometry (1-2ug/million cells)
  • ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Aust et al. The Journal of Immunology, 2009, 183: 106 116

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. ,Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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