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Glutamine Synthetase / GLUL Antibody [MSVA-750M]

In Stock
Catalog Number Formulation Size Price
2752-MSM40-P0
Purified Ab with BSA and Azide
20ug
$324.50
2752-MSM40-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GLUL Antibody in Numerous Tissues (IHC)

GLUL Antibody in Numerous Tissues (IHC)

Glutamine synthetase Mouse Monoclonal Antibody (MSVA-750M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Mitochondria Marker, Neuroinflammation
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Cytosol, Microsome, Mitochondrion
Gene Name
GLUL
Positive Control
Liver: A strong GS staining should be seen of centrilobular hepatocytes while staining is weak to moderate in Kupffer cells.
Immunogen
Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)
Alternate Names
Glutamine synthetase, Glutamate, ammonia ligase, Palmitoyltransferase GLUL, cell proliferation-inducing protein 59; GLUL; Glutamate ammonia ligase; GS; PIG43; PIG59; Glutamate decarboxylase; glutamine synthase; Glutamine synthetase; glutamine synthetase I; Proliferation inducing protein 43; GLNA; GLNS

Database Links

Entrez Gene ID
2752
SwissProt
P15104

Additional Information

Clone
MSVA-750M
Chromosome Location
1q25.3
Mol. Weight of Antigen
42kDa

Related Antibodies

View All GLUL Antibodies

Functions

  • Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:30158707, PubMed:16267323). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351).

Key References

  • Min, Q., et al. 2019. β-catenin and yes-associated protein 1 cooperate in hepatoblastoma pathogenesis. Am. J. Pathol. 189: 1091-1104.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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