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Formalin-fixed, paraffin-embedded human ovarian cancer stained with GCLM Mouse Monoclonal Antibody (GCLM/4069). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified GCLM Mouse Monoclonal Antibody (GCLM/4069). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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g-glutamylcysteine synthetase (g-GCS) is the rate limiting enzyme for glutathione (L-g-glutamyl-L-cysteinylglycine, GSH) synthesis. GSH is ubiquitous in mammalian cells as a vital intra- and extracellular protective antioxidant. g-GCS is a heterodimer of a heavy catalytic subunit and a light regulatory subunit that is responsive to inflammation, phenolic antioxidants, heat shock, oxidants and cytokines. The human g-GCS gene encoding the 367 amino acid catalytic subunit maps to chromosome 6p12. The human g-GCS gene encoding the regulatory subunit maps to chromosome 1p22.1. The two subunits of g-GCS form a heterodimeric zinc metalloprotein that gains activity through formation of a reversible disulfide bond.
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