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Recombinant FOXP1 (Transcription Factor) Antibody [FOXP1/44R]

Additional information

Catalog Number: 27086-RBM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
27086-RBM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
27086-RBM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
27086-RBM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant FOXP1 (Transcription Factor) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with FOXP1 Recombinant Rabbit Monoclonal Antibody (FOXP1/44R) [1ug/ml in PBS] for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant FOXP1 (Transcription Factor) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control. FOXP1/44R at 1ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant FOXP1 (Transcription Factor) Antibody - Image 3

SDS-PAGE Analysis. Purified FOXP1 Recombinant Rabbit Monoclonal Antibody (FOXP1/44R). Confirmation of Integrity and Purity of Antibody.

FOXP1 Antibody in Western Blot (WB)

FOXP1 Antibody in Western Blot (WB)

Western Blot analysis of Ramos cell lysate using FOXP1 Recombinant Rabbit Monoclonal Antibody (FOXP1/44R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FOXP1/44R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
FOXP1
Positive Control
Human tonsil or colon, Ramos
Immunogen
Recombinant fragment (around aa350-450) of human FOXP1 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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, , , ,

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Other Information

Functions
  • Transcriptional repressor (PubMed:18347093, PubMed:26647308). Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential (By similarity). Plays an important role in the specification and differentiation of lung epithelium. Acts cooperatively with FOXP4 to regulate lung secretory epithelial cell fate and regeneration by restricting the goblet cell lineage program; the function may involve regulation of AGR2. Essential transcriptional regulator of B-cell development. Involved in regulation of cardiac muscle cell proliferation. Involved in the columnar organization of spinal motor neurons. Promotes the formation of the lateral motor neuron column (LMC) and the preganglionic motor column (PGC) and is required for respective appropriate motor axon projections. The segment-appropriate generation of spinal cord motor columns requires cooperation with other Hox proteins. Can regulate PITX3 promoter activity; may promote midbrain identity in embryonic stem cell-derived dopamine neurons by regulating PITX3. Negatively regulates the differentiation of T follicular helper cells T(FH)s. Involved in maintenance of hair follicle stem cell quiescence; the function probably involves regulation of FGF18 (By similarity). Represses transcription of various pro-apoptotic genes and cooperates with NF-kappa B-signaling in promoting B-cell expansion by inhibition of caspase-dependent apoptosis (PubMed:25267198). Binds to CSF1R promoter elements and is involved in regulation of monocyte differentiation and macrophage functions; repression of CSF1R in monocytes seems to involve NCOR2 as corepressor (PubMed:15286807, PubMed:18799727, PubMed:18347093). Involved in endothelial cell proliferation, tube formation and migration indicative for a role in angiogenesis; the role in neovascularization seems to implicate suppression of SEMA5B (PubMed:24023716). Can negatively regulate androgen receptor signaling (PubMed:18640093). Acts as a transcriptional activator of the FBXL7 promoter; this activity is regulated by AURKA (PubMed:28218735).
  • Involved in transcriptional regulation in embryonic stem cells (ESCs). Stimulates expression of transcription factors that are required for pluripotency and decreases expression of differentiation-associated genes. Has distinct DNA-binding specifities as compared to the canonical form and preferentially binds DNA with the sequence 5'-CGATACAA-3' (or closely related sequences) (PubMed:21924763). Promotes ESC self-renewal and pluripotency (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
27086
SwissProt
Q9H334
Chromosome Location
3p13
Mol. Weight of Antigen
75kDa
Alternate Names
Forkhead box protein P1, Mac-1-regulated forkhead, Fork head related protein like B; Forkhead box protein P1 (FOXP1); Glutamine rich factor 1; hFKH1B; HSPC215; QRF1

Summary

Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development. FOXP1 protein plays an important role in the specification and differentiation of lung epithelium, also acts cooperatively with FOXP4 to regulate lung secretory epithelial cell fate and regeneration by restricting the goblet cell lineage program. FOXP1 is useful in subclassification of DLBCL and a high cutoff ( � �80%) for FOXP1 is needed to achieve high specificity for the ABC subtype.

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