Fibroblast Activation Protein Alpha / FAP-1 Antibody [FAP/4851]

These antibodies are validated on HuProtâ„¢ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 2191-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
2191-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2191-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2191-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Host
Mouse
Applications
Species Reactivity
Clone
FAP/4851
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell projection, Cell surface, Cytoplasm, Invadopodium membrane, Lamellipodium membrane, Membrane, Ruffle membrane, Secreted
Gene Name
FAP
Positive Control
colon or pancreas. Fibroblast specific., Human uterus
Immunogen
Recombinant fragment (around aa 1-200) of human FAP protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
2q24.2
Mol. Weight of Antigen
88kDa
Alternate Names
Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, 170 kDa melanoma membrane-bound gelatinase; DPPIV; FAP; FAPA; Fibroblast activation protein alpha; Integral membrane serine protease; Seprase

Summary

FAP (fibroblast activation protein) is a cell surface glycoprotein and serine protease that is expressed primarily in fetal mesenchymal tissues and epithelial cancer fibroblasts. In cancer, FAP functions to promote cellular proliferation. In embryonic development, FAP functions to remodel developing tissues. FAP acts as an integral membrane gelatinase composed of N-glycosylated proteolytically inactive subunits. FAP expression on chondrocyte membranes is upregulated by the combination of the cytokines IL-1 and OSM and has been shown to increase in osteoarthritic patients. This expression is colocalized with MMP-1 and MMP-13 as well as CD44 (variants v3 and v7/8). Mice that lack all copies of the FAP gene have been found to be fertile and to have developmental defects or change in cancer susceptibility.

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