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EZH2 / KMT6 Antibody [EZH2/2536]

In Stock
Catalog Number Formulation Size Price
2146-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2146-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2146-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol
Western Blot (WB)
2-4ug/ml

Summary

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Nucleus
Gene Name
Positive Control
HeLa, MCF7, testis or prostate tissue., U-2 OS or HEK-293 whole cell lysates. Human tonsil
Immunogen
Recombinant full-length human EZH2 protein
Alternate Names
Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, Enhancer of zeste 2 polycomb repressive complex 2 subunit; Enhancer of zeste homolog 2 (EZH2); ENX1; Enx1h; EZH2b; Histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; Lysine N-methyltransferase 6; WVS; WVS2

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
EZH2/2536
Chromosome Location
7q36.1
Mol. Weight of Antigen
81-102kDa

Functions

  • Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599, PubMed:30923826). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.

Key References

  • Yamagishi M et al. Curr Opin Oncol. 2017 Sep;29(5):375-381.
  • Han Li C et. al. Curr Protein Pept Sci. 2015;16(6):559-70.
  • Shinozaki-Ushiku, A. et al. Histopathology 2017; 70, 722-733.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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