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Ep-CAM / CD326 (Extracellular Domain) (Epithelial Marker) Antibody [Ber-EP4]

Additional information

Catalog Number: 4072-MSM4-IHC7
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Best Seller
Catalog Number Formulation Size Price
4072-MSM4-IHC7
7.0ml of Prediluted Antibody for IHC
7.0ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EpCAM Antibody in Immunohistochemistry (IHC (P))

EpCAM Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon stained with EpCAM Mouse Monoclonal Antibody (Ber-EP4).

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
Ber-EP4
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
MCF-7 or HT29 cells. Breast tumor.
Immunogen
Human breast cancer MCF-7 cells
Purification
Protein A
Storage & Stability
Store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
7.0ml of Ready-to-Use for IHC staining of formalin-fixed paraffin-embedded tissues
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Key References
  • Latza U, Niedobitek G, Schwarting R, Nekarda H, Stein H. Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelia. J Clin Pathol 1990;43:213-9.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4072
SwissProt
P16422
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Summary

Recognizes a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. It has been reported that this epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

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