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Desmoglein-3 (Squamous Cell Marker) Antibody [DSG3/2838]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
1830-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1830-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1830-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Research has shown that DSG3 has a very high sensitivity (80%) and specificity (100%) in recognizing squamous cell carcinoma (SqCC). Therefore, DSG3 is considered a very important marker for lung SqCC and can be a useful ancillary marker to separate SqCC from other subtypes of lung cancer. Moreover, studies have shown that DSG3 expression in lung SqCC may indicate a poor prognosis.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Desmosome
Gene Name
Positive Control
A431 cells. Skin or Lung Squamous Cell Carcinoma (SCC).
Immunogen
Recombinant fragment (around aa 379-491) human DSG3 protein (exact sequence is proprietary)
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
DSG3/2838
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Key References

  • Savci-Heijink CD, et al. The role of desmoglein-3 in the diagnosis of squamous cell carcinoma of the lung. Am J Pathology 2009; 174(5):1629-37.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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