These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE
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Immunofluorescent analysis of PFA-fixed MCF-7 cells. DDX41 Mouse Monoclonal Antibody (PCRP-DDX41-1B4) followed by goat anti-mouse IgG-CF488 (green); counterstain is phalloidin (red).
Flow cytometric analysis of PFA-fixed HeLa cells. DDX41 Mouse Monoclonal Antibody (PCRP-DDX41-1B4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
SDS-PAGE Analysis of Purified DDX41 Mouse Monoclonal Antibody (PCRP-DDX41-1B4). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using DDX41 Mouse Monoclonal Antibody (PCRP-DDX41-1B4). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol |
|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol |
| Immunofluorescence (IF) | 1-3ug/ml | |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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DDX41 (Probable ATP-dependent RNA helicase DDX41, DEAD box protein abstrakt homolog) is a 622 amino acid protein encoded by the human gene DDX41. DDX41 belongs to the DEAD box helicase family (DDX41 subfamily) and contains one CCHC-type zinc finger, one helicase ATP-binding domain and one helicase C-terminal domain. DDX41 is required during post-transcriptional gene expression and is thought to be involved in pre-mRNA splicing. DDX41 is believed to be a probable ATP-dependent RNA helicase. RNA helicases are highly conserved enzymes that utilize the energy derived from NTP hydrolysis to modulate the structure of RNA. RNA helicases participate in all biological processes that involve RNA, including transcription, splicing and translation.
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