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Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody [KRT7/3733]

Additional information

Catalog Number: 3855-MSM13
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3855-MSM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3855-MSM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3855-MSM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/3733). Inset: PBS instead of primary antibody; secondary only negative control.

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/3733). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/3733). Confirmation of Purity and Integrity of Antibody.

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) Antibody - Image 4

Western blot analysis of SK-BR3 cell lysate using Cytokeratin-7 Mouse Monoclonal Antibody (KRT7/3733).

Cytokeratin 7 Antibody in Western Blot (WB)

Cytokeratin 7 Antibody in Western Blot (WB)

Western blot analysis of human lung tissue lysates and A549 cell lysates using Cytokeratin 7 Mouse Monoclonal Antibody (KRT7/3733).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
KRT7/3733
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
KRT7
Positive Control
Cervix|HeLa or SK-BR3 cells. Carcinoma of ovary|lung|or breast (IHC).
Immunogen
Recombinant full-length human KRT7 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
Key References
  • Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 1990;136:641-55
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3855
SwissProt
P08729Human
Chromosome Location
12q13.13
Mol. Weight of Antigen
55kDa
Alternate Names
Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7

Summary

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. MAb to cytokeratin 7 is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

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