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Cytokeratin 14 (KRT14) (Squamous Cell Marker) Antibody [KRT14/4125]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
3861-MSM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3861-MSM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3861-MSM5-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells.Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors.Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
Positive Control
A431 orHeLacells. Skin or squamous cell carcinoma.
Immunogen
Recombinant human KRT14 fragment (around aa351-472) (exact sequence is proprietary)
Alternate Names
Keratin, type I cytoskeletal 14, Cytokeratin-14, Keratin-14, CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
KRT14/4125
Chromosome Location
17q21.2
Mol. Weight of Antigen
~50kDa

Functions

  • The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Key References

  • Leigh I.M.,et al. (1988). Dermatology, 91:415.
  • Purkis, P., et al. 1990. Journal of Cell Science. 97: p39-50.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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