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Creatine Kinase-BB (CK-BB) Antibody [2ba6]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
1152-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1152-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1152-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. The specificity of this monoclonal antibody to its intended target was validated by HuProt Array containing more than 19,000 full-length, correctly-folded human proteins. Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm, Cytosol, Mitochondrion
Gene Name
CKB
Positive Control
Cerebellum.
Immunogen
Human CKBB protein
Alternate Names
Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; CKBB creatine kinase B-type; creatine kinase, brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
2ba6
Chromosome Location
14q32
Mol. Weight of Antigen
43kDa (Monomer); 86kDa (Dimer)

Functions

  • Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).

Key References

  • Mariman, E.C., et al. 1987. Structure and expression of the human creatine kinase B gene. Genomics 1: 126-137.
  • Mariman, E.C., et al. 1989. Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17: 6385.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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