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Connexin 32 (Gap Junction Protein) Antibody [M12.13]

In Stock
Catalog Number Formulation Size Price
2705-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2705-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2705-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG /
Cellular Localization
Cell junction, Cell membrane, Gap junction
Gene Name
Positive Control
Kidney, MCF-7 cells. Liver, Stomach or Tonsil.
Immunogen
Rat junctional complexes
Alternate Names
Gap junction beta-1 protein, Connexin-32, GAP junction 28 kDa liver protein, Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
M12.13
Chromosome Location
Xq13.1
Mol. Weight of Antigen
27-32kDa

Functions

  • One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Key References

  • D. A. Goodenough et. al. Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions. Journal of Cell Biology, 107(5), 1817-1824 (1988)

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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