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CD9 (TSPAN29) (Motility-Related Protein-1) Antibody [CD9/1619]

Additional information

Catalog Number: 928-MSM1
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
928-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
928-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
928-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD9 (TSPAN29) (Motility-Related Protein-1) Antibody

Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with CD9 Mouse Monoclonal Antibody (CD9/1619).

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Tonsil stained with CD9 Mouse Monoclonal Antibody (CD9/1619).

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 3

Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD9 Mouse Monoclonal Antibody (CD9/1619).

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 4

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CD9 Mouse Monoclonal Antibody (CD9/1619).

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 5

SDS-PAGE Analysis Purified CD9 Mouse Monoclonal Antibody (CD9/1619). Confirmation of Integrity and Purity of Antibody.

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 6

Flow Cytometry Analysis of MCF-7 cells unstained (gray) or stained with CD9 monoclonal antibody (CD9/1619) followed by goat anti mouse CF680 (red).

CD9 (TSPAN29) (Motility-Related Protein-1) Antibody - Image 7

Flow cytometry of bead-bound exosomes derived from MCF-7 cells. Unstained exosomes (gray) or stained with CF568-labeled CD9 monoclonal antibody (CD9/1619) (orange).

CD9 Antibody in Western Blot (WB)

CD9 Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain and Rabbit Brain tissue lysates using CD9 Mouse Monoclonal Antibody (CD9/1619).

  • Flow
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CD9/1619
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Extracellular exosome, Membrane, Secreted
Gene Name
CD9
Positive Control
BT-20, HeLa or ZR-75 cells. Cervix or Esophagus., MCF-7 or Human Brain
Immunogen
Recombinant full-length human CD9 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Gene:

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Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:8478605, PubMed:14575715, PubMed:18541721). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:8478605, PubMed:7511626).
Key References
  • Lanza, F., et al. 1991. cDNA cloning and expression of platelet p24/CD9. Evidence for a new family of multiple membrane-spanning proteins. J. Biol. Chem. 266: 10638-10645.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
928
SwissProt
P21926
Chromosome Location
12p13.31
Mol. Weight of Antigen
24kDa
Alternate Names
CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, Tetraspanin-29 (TSPAN29); BA-2/p24 antigen; BA2; BTCC1; CD9; Cell growth-inhibiting gene 2 protein (GIG2); DRAP27; Leukocyte antigen MIC3; Motility-related protein (MRP1); p24

Summary

CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.

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