Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Tonsil stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD73 Mouse Monoclonal Antibody (NT5E/2503).
Immunofluorescence staining of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).
Flow Cytometric Analysis of U87MG cells using CD73 Mouse Monoclonal Antibody (NT5E/2503) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western blot analysis of A-431 and PC3 cell lysate using CD73 Mouse Monoclonal Antibody (NT5E/2503).
SDS-PAGE Analysis of Purified CD74 Mouse Monoclonal Antibody (NT5E/2503). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of Human Liver, Human Skin, and Human Spleen tissue lysates using CD73 Mouse Monoclonal Antibody (NT5E/2503).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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CD73 (also designated ecto-5′-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
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