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CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody [NK1/7566]

Additional information

Catalog Number: 27087-MSM6
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
27087-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
27087-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
27087-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody

SDS-PAGE Analysis of Purified CD57 Mouse Monoclonal Antibody (NK1/7566). Confirmation of Purity and Integrity of Antibody.

CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody - Image 2

Western blot analysis of Daudi cell lysate using CD57 Mouse Monoclonal Antibody (NK1/7566).

CD57 / B3GAT1 (Natural Killer Cell Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human lymph node stained with CD57 Mouse Monoclonal Antibody (NK1/7566). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
NK1/7566
Isotype
IgG2
Light Chain
Lambda
Cellular Localization
Cell surface
Gene Name
B3GAT1
Positive Control
Human lymph node or tonsil.
Immunogen
Recombinant fragment (around aa1-200) of human B3GAT1 / CD57 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Key References
  • Okada, T., et al. 1995. Origin of CD57+ T cells which increase at tumour sites in patients with colorectal cancer. Clin. Exp. Immunol. 102: 159-166.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
27087
SwissProt
Q9P2W7
Chromosome Location
11q25
Mol. Weight of Antigen
~110kDa
Alternate Names
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Beta-1, 3-glucuronyltransferase 1, Glucuronosyltransferase P, UDP-GlcUA:glycoprotein beta-1, 3-glucuronyltransferase, 3-Glucuronyltransferase 1; B3GAT1; Galactosylgalactosylxylosylprotein 3-beta-Glucuronosyltransferase 1; GLCATP; GlcUAT-P; Glucuronosyltransferase P; UDP GlcUA Glycoprotein beta 1, 3 Glucuronyltransferase

Summary

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

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