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Formalin-fixed, paraffin-embedded human Tonsil (10X) stained with CD45 Mouse Monoclonal Antibody (2B11).
Formalin-fixed, paraffin-embedded human Tonsil (20X) stained with CD45 Mouse Monoclonal Antibody (2B11).
SDS-PAGE Analysis of Purified CD45 Mouse Monoclonal Antibody (2B11). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of live Jurkat cells unstained (grey) and using CD45 Mouse Monoclonal Antibody (2B11) conjugated with CF680 (purple).
Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF568-labeled CD45 monoclonal antibody (2B1) (green).
SDS-PAGE Analysis of Purified Receptor-type tyrosine-protein phosphatase C Recombinant Mouse Monoclonal Antibody (2B11). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
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CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
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