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CD21 (Mature B-Cell & Follicular Dendritic Cell Marker) Antibody [CR2/6683]

In Stock
Catalog Number Formulation Size Price
1380-MSM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1380-MSM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1380-MSM13-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded Human Tonsil stained with CD21 Mouse Monoclonal Antibody (CR2/6683). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded Human Tonsil stained with CD21 Mouse Monoclonal Antibody (CR2/6683). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using CR2/6683 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

IHC analysis of formalin-fixed, paraffin-embedded human testis. Negative tissue control using CR2/6683 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Research Areas
Immunology, B Cell Markers, Complement System, Dendritic Cell Marker
Isotype / Light Chain
IgG2a / Kappa
Cellular Localization
Cell surface
Gene Name
CR2
Positive Control
Raji or WEHI-231 cells. Tonsil
Immunogen
human B cell line SB
Alternate Names
Complement receptor type 2, Complement C3d receptor, Epstein-Barr virus receptor, CD21; Complement C3d receptor (C3DR); Complement Receptor type 2 (CR2); CVID7; EBV receptor; EBV-R; Epstein-Barr virus receptor; EVBR; SLEB9

Database Links

Entrez Gene ID
1380
SwissProt
P20023Human

Additional Information

Clone
CR2/6683
Chromosome Location
1q32
Mol. Weight of Antigen
140kDa

Related Antibodies

View All CR2 Antibodies

Functions

  • Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).
  • (Microbial infection) Acts as a receptor for Epstein-Barr virus.

Key References

  • Otsuka, M., et al. 2004. Role of CD21 antigen in diffuse large B-cell lymphoma and its clinical significance. Br. J. Haematol. 127: 416-424.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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