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CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody [BH1]

Additional information

Catalog Number: 914-MSM3-CF488-100T
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
914-MSM3-CF488-100T
Purified Ab Conjugated to CF488
0.5ml at 100ug/ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody

SDS-PAGE Analysis of Purified CD2 Mouse Monoclonal Antibody (BH1). Confirmation of Integrity and Purity of Antibody.

CD2 / Lymphocyte Function Antigen 2 (LFA-2) Antibody - Image 2

Flow Cytometric Analysis of MOLT-4 cells using CD2 Mouse Monoclonal Antibody (BH1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Flow
  • Functional Studies
  • IF
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Functional Studies, Immunofluorescence
Species Reactivity
Human
Clone
BH1
Isotype
IgG2b
Cellular Localization
Cell membrane, Cell surface
Gene Name
CD2
Positive Control
293T or Jurkat cells. Lymph node and tonsil.
Immunogen
Human CD2 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Key References
  • Wang MY et. al. An effective immuno-magnetic method for bone marrow purging in T cell malignancies. Bone Marrow Transplant. 1992;9(5):319-23.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
914
SwissProt
P06729
Chromosome Location
1p13.1
Mol. Weight of Antigen
~50kDa
Alternate Names
T-cell surface antigen CD2, Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, T-cell surface antigen T11/Leu-5, Erythrocyte receptor; LFA-2; LFA-3 receptor; Ly-37; Lymphocyte function antigen 2; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11

Summary

CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.

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