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Recombinant CD171 / NCAM-L1 (L1 Cell Adhesion Molecule) Antibody [L1CAM/9267R]

In Stock
Catalog Number Formulation Size Price
3897-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3897-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3897-RBM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm. Cell surface.
Gene Name
Positive Control
Human brain total lysate (WB). PC12 and Neuro-2A (ICC).
Immunogen
Recombinant fragment (around aa600-900) of human L1CAM protein (exact sequence is proprietary)
Alternate Names
Neural cell adhesion molecule L1, CAML1, HSAS1, Hyd, L1 Cell Adhesion Molecule, L1-NCAM, MASA, MIC5, NCAM-L1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, S10, SPG1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
L1CAM/9267R
Chromosome Location
Xq28
Mol. Weight of Antigen
full-length isoforms: 140/180/220kDa; proteolytically cleaved form:85 kDa

Functions

  • Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Key References

  • Neuron.1989 Jun; 2(6):1597-603.
  • J of Cell Biol 2002 Jun; 157(7):1223-1232.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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