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CD106 / VCAM1 (Activated Endothelial Cell Marker) Antibody [1.4C3]

In Stock
Catalog Number Formulation Size Price
7412-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
7412-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
7412-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified CD106 Monoclonal Antibody (1.4C3). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified CD106 Monoclonal Antibody (1.4C3). Confirmation of Purity and Integrity of Antibody.

Formalin-fixed, paraffin-embedded human Tonsil stained with CD106 Monoclonal Antibody (1.4C3).

Formalin-fixed, paraffin-embedded human Tonsil stained with CD106 Monoclonal Antibody (1.4C3).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 110kDa, identified as CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100). CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular and inter-follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Hypoxia, Immunology, BBB VCAM-1 Signaling, Cytokine Signaling, Endothelial Cell Marker, Mesenchymal Stem Cell Differentiation, Stem Cell Differentiation
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Membrane
Gene Name
VCAM1
Positive Control
Human placenta or tonsil.
Immunogen
Stimulated human umbilical vein endothelial cells (HUVEC)
Alternate Names
Vascular cell adhesion protein 1, INCAM-100, CD106; INCAM-100; L1CAM; Vascular Cell Adhesion Molecule 1 (VCAM1); Vascular cell adhesion protein 1

Database Links

Entrez Gene ID
7412
SwissProt
P19320

Additional Information

Clone
1.4C3
Chromosome Location
1q21.2
Mol. Weight of Antigen
110kDa

Related Antibodies

View All VCAM1 Antibodies

Functions

  • Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Key References

  • Vermot-Desroches C et al. Heterogeneity of antigen expression among human umbilical cord vascular endothelial cells: identification of cell subsets by co-expression of haemopoietic antigens. Immunol Lett 1995, 48(1):1-9
  • Rice GE et al. Vascular and nonvascular expression of INCAM-110. A target for mononuclear leukocyte adhesion in normal and inflamed human tissues. Am J Pathol 1991, 138(2):385-393
  • Huang MJ et al. Expression of vascular cell adhesion molecule-1 by follicular dendritic cells. Leuk Lymphoma 1995, 18(3-4):259-264.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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