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Carbonic Anhydrase 3 / CA3 Antibody [CA3/7883]

Additional information

Catalog Number: 761-MSM3
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
761-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
761-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
761-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Carbonic Anhydrase 3 / CA3 Antibody

SDS-PAGE Analysis of Purified CA3 Mouse Monoclonal Antibody (CA3/7883). Confirmation of Purity and Integrity of Antibody.

Carbonic Anhydrase 3 / CA3 Antibody - Image 2

Formalin-fixed, paraffin-embedded human skeletal muscle stained with CA3 Mouse Monoclonal Antibody (CA3/7883). Inset: PBS instead of primary antibody; secondary only negative control.

Carbonic Anhydrase 3 / CA3 Antibody - Image 3

Western blot analysis of RH-30 cell lysate using CA3 Mouse Monoclonal Antibody (CA3/7883).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CA3/7883
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
CA3
Positive Control
Muscle specific.
Immunogen
Recombinant fragment of human CA3 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Reversible hydration of carbon dioxide.
Key References
  • Heath, R., et al. 1985. Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase. Am. J. Med. Genet. 21: 291-296
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
761
SwissProt
P07451Human
Chromosome Location
8q21.2
Mol. Weight of Antigen
28kDa
Alternate Names
Carbonic anhydrase 3, Carbonate dehydratase III, Carbonic anhydrase III, Apotransferrin; Beta-1 metal-binding globulin; Serotransferrin; Siderophilin; CA3; CA3QTL1; CA3

Summary

Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.

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