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Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with BMI1 Mouse Monoclonal Antibody (BMI1/2823).
Western Blot Analysis of Human NIH3T3 cell lysate using BMI1 Mouse Monoclonal Antibody (BMI1/2823).
SDS-PAGE Analysis Purified BMI1 Mouse Monoclonal Antibody (BMI1/2823). Confirmation of Integrity and Purity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling BMI1 with BMI1 Mouse Monoclonal Antibody (BMI1/2823) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.
Analysis of Protein Array containing more than 19,000 full-length human proteins using BMI1 Mouse Monoclonal Antibody (BMI1/2823) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÂ’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÂ’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
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