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Recombinant Biotin (Vitamin B7 or Vitamin H) Antibody [BTN/8672R]

Additional information

Catalog Number: RBM7-8672
Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
RBM7-8672-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
RBM7-8672-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
RBM7-8672-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Biotin (Vitamin B7 or Vitamin H) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with Biotin Rabbit Recombinant Monoclonal antibody (BTN/8672R).HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Biotin Antibody in SDS-PAGE

Biotin Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified BTN Recombinant Rabbit Monoclonal Antibody (BTN/8672R). Confirmation of Purity and Integrity of Antibody.

Biotin Antibody in Western Blot (WB)

Biotin Antibody in Western Blot (WB)

Western blot analysis of human kidney and human liver tissue lysates using Biotin Recombinant Rabbit Monoclonal Antibody (BTN/8672R).

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
All species
Clone
BTN/8672R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Depends on the target
Positive Control
Biotinylated proteins in solution or on tissues, kidney, liver
Immunogen
Biotinylated sheep immunoglobulin
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis

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Other Information

Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Applicable
SwissProt
Not Applicable
Mol. Weight of Antigen
50-244Da
Alternate Names
, Vitamin B7 or Vitamin H

Summary

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

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Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

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