Biotin (Vitamin B7 or Vitamin H) [BTN/403]

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Summary

It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
244Da
Immunogen
Biotinylated sheep immunoglobulin

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
BTN/403
Human Gene Symbol
Not Applicable
Chromosome Location
Not Applicable

Known Applications & Suggested Dilutions

  • Confocal Microscopy
  • In situ Nucleic Acid Hybridization
  • Flow Cytometry (0.5-1ug/million cells)
  • ,Immunofluorescence (0.5-1ug/ml)
  • Western Blot (0.5-2ug/ml)
  • ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Uccellini MB,et al.2008.J. Immunol.181:5875.
  • Green NM,et al.2012.J Biol Chem.287:39789

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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