Alpha-1-Antitrypsin (SERPINA1) (Hepatocellular & Histiocytic Marker) Antibody [AAT/4486]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 5265-MSM16
Host:

Reactivity:

, ,

Gene:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
5265-MSM16-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5265-MSM16-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5265-MSM16-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • IHC
  • WB

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Host
Mouse
Species Reactivity
Clone
AAT/4486
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
hepatocellular carcinoma or histiocytoma (IHC).|Human tonsil,Human Lung, Mouse Lung or Rat Lung
Immunogen
Recombinant fragment (around aa200-400) of human SERPINA1 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
SwissProt
Chromosome Location
14q32.1
Mol. Weight of Antigen
46kDa
Alternate Names
Alpha-1-antitrypsin | Short peptide from AAT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, Serpin A1, A1AT; Alpha-1 protease inhibitor; Alpha-1-antiproteinase (Alpha1AT); Dom1; Serine (or cysteine) proteinase inhibitor clade A member 1; Serpin peptidase inhibitor clade A member 1; Short peptide from AAT (SPAAT); Spi1-1

Summary

It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

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